12/7/2023 0 Comments Western blot results![]() ![]() This is especially true when the disease has reached the chronic stage. The problem with Lyme disease is that the bacteria can create a hard “shell” around themselves when inactive so that the blood tests are unable to detect them. Lyme Disease is Remarkably Difficult to Diagnose At this point, a medical provider will order a Western blot test to confirm the presence of Lyme disease. If any of these tests come back as positive, then other samples will be used to track the stage of the disease to determine if it has reached the chronic infection stage. This blood test does not always detect the presence of the disease, so patients that have persistent symptoms after having the test may be re-tested in a few weeks. Recent infections are much easier to detect and an IgM and IgG blood test will often be ordered as complimentary information gathering tools. ![]() It's a particular problem with blots exposed on film where there can be saturation of the film exposure, maybe less so with more recent fluorescence-based approaches.The Lyme disease blood test is used to discover if someone who has the symptoms of a Borrelia burgdorferi infection actually has the bacteria in their bloodstream. That difference among biologic replicates is the variability against which you should statistically evaluate any differences you observe.įurthermore, quantifying Western blots can have serious pitfalls, depending on how it's done. You will almost always find that the variability among experiments on biologic replicates is much greater than the within-experiment errors from loading, blotting, exposing, and reading the samples. You really have to do the whole experiment several times, starting from fresh cells or tissues, to have a biologically reliable result. Please don't believe any results based on a single biologic replicate. And re-running the same protein extracts on another gel doesn't count as a biologic replicate that would simply account for the random vagaries of gel loading, antibody binding, film exposure conditions, etc. Re-reading the densitometry results would help account for some of the random vagaries of densitometry, but it would not get at the more fundamental and potentially much larger source of error: the differences among true biologic replicates of the same experiment. ![]() The main problem you have is what you fear: you can't do proper statistical analysis on a single observation. Please excuse me if the questions are too obvious or foolish. I'm a medical student and as is apparent, my stats are a little weak. Values for the control group are going to be 1.
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